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. 2016 Nov 1;143(21):3944–3955. doi: 10.1242/dev.138917

Fig. 5.

Fig. 5.

Rusc2 induces cytosolic Gli protein aggregates. (A) Western blot showing that overexpression of hSUFU, but not hRUSC2, stabilizes Gli2 and Gli3 in NIH3T3 cells. (B) Dual-luciferase assay showing that hSUFU and hRUSC2 reduce the activity of Gli1 and Gli2. Compared with hSUFU, hRUSC2 was less potent in this assay. Data are shown as mean±s.d. **P<0.01. (C) Immunofluorescence showing the effects of hSUFU and hRUSC2 on the subcellular localization of myc-Gli3 in NIH3T3 cells. Left, cells without Triton X-100 extraction; right, cells extracted with Triton X-100 prior to fixation. (D) Immunofluorescence showing that overexpression of hRUSC2 alters the subcellular distribution of myc-Gli3 in wild-type MEFs (left), but not that in Sufu knockout MEFs (right). Scale bars: 10 µm.