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. 2016 Nov 1;143(21):3933–3943. doi: 10.1242/dev.139253

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© 2016. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Fig. 3. — Super-resolution microscopy and transmission electron microscopy show collagen I on ECs in developing bones. (A,B) STORM images of cross-sections of humeri at E15.5 immunostained for EMCN (red) and collagen I (green). (B) A side view of the boxed area in A. (C,D) Cross-sections of E16.5 mouse humerus observed by TEM. (C) Cross-sections of blood vessels from the bone area. (D) Magnification of the boxed area in C. Collagen type I immunostained with 10 nm gold particles is located on the endothelial cell (EC) surface. RBC, red blood cells. Scale bars: 1 µm in A; 800 nm in B; 1 µm in C; 200 nm in D.