Fig. 5.

Several Khc-dependent processes are unaffected upon Tm1C depletion. (A–C) Ovaries were fixed from flies expressing either a control shRNA (A), khc shRNA (B) or Tm1 shRNA-2 (C) and were counterstained with TRITC–phalloidin to visualize F-actin. (D–F) Ovaries were dissected from the same strains used in A–C. The egg chambers were fixed and processed using an antibody against Lamin DmO (green). Arrows indicates the oocyte nucleus. (G–I) Ovaries from flies expressing a control shRNA (G), khc shRNA (H) or Tm1 shRNA-2 (I) were processed for in situ hybridization using probes against grk mRNA (green). The egg chambers were counterstained with ToPro3 (red). (J–L) The same strains were fixed and processed for in situ hybridization using probes against bcd mRNA (green). The egg chambers were counterstained with ToPro3 (red). (M,N) Embryos were collected and fixed from mothers expressing control shRNA (M) or Tm1 shRNA-2 (N). The embryos were processed for in situ hybridization using probes against nanos mRNA (nos, green). M′ and N′ represent blastoderm stage egg chambers from the indicated strains. The posterior region of these embryos is shown. (O–P) Embryos from these same strains were fixed and processed for in situ hybridization using probes against Cyclin B mRNA (cycB, green). As with the above panel, O′ and P′ represent blastoderm stage egg chambers from the indicated strains. Quantification of phenotypes and the number of egg chambers or embryos scored are indicated. Scale bars: 50 μm (A–P); 25 μm (M′,N′,O′,P′).