Fig. 1.

Yeast SOD1 ALS mutant alleles. (A) Amino acid residues commonly substituted within ALS patients and that were mutated in this study are conserved between yeast and human Sod1 (left). Their distribution is indicated upon the crystal structure of a human Sod1 dimer (PDB ID: 1HL4) (right). (B–E) Wild-type or cells deleted for SOD1 and re-expressing SOD1 or sod1 mutants as indicated were assayed for protein level by western blotting during logarithmic (B) and stationary phase (D) of growth or for enzymatic activity during logarithmic (C) or stationary phase (E) of growth.