Fig. 4.

The importin-β inhibitor importazole blocks nuclear import of CRABP2. M2^−/− cells were transfected with a vector encoding Flag–CRABP2. Cells were pretreated with importazole (30 µM) for 1 h prior to treatment with RA. Flag–CRABP2 (red) was immunostained and visualized using confocal microscopy. DAPI was used to visualize nuclei.