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. 2016 Nov 1;129(21):4046–4056. doi: 10.1242/jcs.192211

Fig. 4.

Fig. 4.

Rnd3 C-terminus and effector domains are required for plexin-B2 interaction. (A) Schematic representation of Rnd3 mutants used in this study. Full-length Rnd3 has N- and C-terminal extensions and a core GTP-binding region (residues 16–200). Rnd3ΔC contains amino acids 1–200, Rnd3ΔN amino acids 16–244 and Rnd3ΔNΔC amino acids 16–200. Point mutants are shown below. (B) Structure prediction analysis of the key residues involved in Rnd3 interaction with plexin-B RBD is represented as a ribbon diagram identifying Rnd3 effector domain and non-effector domain amino acids. Plexin-B1 RBD is shown in pink, Rnd1 in yellow (PDB 2REX), Rnd3 in cyan. (C–E) Lysates of COS7 cells expressing HA–plexin-B2-cyto and FLAG–Rnd1, FLAG–Rnd3 mutants or FLAG–RhoA-G14V were immunoprecipitated with anti-HA antibody followed by immunoblotting with the indicated antibodies. All blots are representative of three independent experiments; * indicates IgG or nonspecific bands.