Fig. 1.

miR-501 is highly enriched in activated myogenic progenitors. TA muscles were injected with CTX and analyzed 3 days later. (A) MPs and FAPs were identified based on their distinct expression pattern of SCA1 and alpha 7-integrin. Orange: PE⁺ gate; green: APC⁺ gate. (B) Mice were injected intraperitoneally with 10 µg EdU per gram body weight 12 h before harvesting. Proliferation was measured by EdU incorporation assay using FACS. Shown are cell counts based on Alexa Fluor 647 (AF647) staining. (C) Results of small RNA deep sequencing, comparing activated MPs and FAPs, n=2, each sample representing a pool of cells obtained from seven to nine mice for MPs and two mice for FAPs. The five miRNAs that were identified as being highly expressed and at least fivefold enriched in MPs versus FAPs are marked.