Fig. 3.
miR-501 is a conserved intronic miRNA residing in the Clcn5 gene, isoform-2. (A) Schematic of mouse (top) and human (bottom) miR-501 locus and its host gene, Clcn5. The intron located between two isoform 2-specific exons harbors a cluster of conserved miRNAs. Binding sites for isoform-specific (F1, F2, R1,2) and common primers (FC, RC) used for mouse transcripts are indicated. (B) Alignment of mouse and human miR-501-3p sequences. The seed region is highlighted. (C) Relative expression of the miRNAs clustered in intron 2 of mouse Clcn5 in MPs and FAPs sorted from CTX-injected TA muscle, as shown in Fig. 1. Data are the mean±s.e.m.