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. 2016 Nov 15;143(22):4137–4148. doi: 10.1242/dev.136051

Fig. 7.

Fig. 7.

miR-501 is increased in skeletal muscle from mdx mice and is a marker for disease severity in serum. (A,B) Expression of miR-501 and myomiRs in TA muscle (A) or serum (B) from 12-week-old C57BL/6 and mdx mice as measured by qRT-PCR. Data are normalized to sno234 (n=3; A) or to the spiked cel-miR-39 (n=5-9; B). (C-G) Creatine kinase (CK) activity and expression of miR-501 and myomiRs in serum from mdx mice at different ages. miRNA expression levels were measured by qRT-PCR and normalized to the spiked cel-miR-39. n=3-10 per time point. (H) Hypothetical model for the expression and function of miR-501 during regeneration of adult muscle tissue. In MPs, the miR-501 cluster is transcribed together with Clcn5 isoform-2. Muscle regeneration enhances the cleavage of the miR-501 cluster leading to increasing miR-501 concentrations in activated MPs and neofibers. In neofibers, miR-501 regulates myosin formation through gigaxonin and potentially other targets. Data are presented as mean±s.e.m. *P<0.05, **P<0.01, ***P<0.001, Student's t-test.