Fig. 4.

Impact of maternal loading for GALK on survival of larvae to adulthood in the presence versus absence of 200 mM dietary galactose. As indicated, GALT+, GALK-null larvae that received maternally loaded GALK survived to a greater extent in both the absence and presence of dietary galactose (gal) than did their counterparts that did not receive GALK maternal loading (P<0.0001 for both comparisons). In contrast, loss of maternally loaded GALK compromised survival of GALT-null GALK-null larvae by close to three-fold in the absence of galactose (P<0.0001) and by close to 10-fold in the presence of galactose (P=0.0005). NA, not applicable. Values plotted represent mean±s.e.m.; the number of cohorts tested for each genotype (n) is indicated on the figure. P-values were calculated using an effects model, as described in Materials and Methods, and a t-test for pair-wise comparisons.