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. 2016 Nov 1;9(11):1405–1417. doi: 10.1242/dmm.026658

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© 2016. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Fig. 3. — Expression of epithelial markers in mMEC cultures. (A) End-point RT-PCR showing expression of a selected panel of upper airway-associated genes in mMEC original cells isolated from the middle ear cavity, ALI day 0 cells and ALI day 14 cells, with fibroblasts as a negative control. Expression profile of ALI day 14 cells was similar to mMEC original cells isolated from the middle ear for most genes. (B-D) Detection of Bpifa1 (B), lactotransferrin (C) and Reg3γ (D) in the apical washes from differentiating cells using western blotting. Data is representative of three independent cultures.