Table 1.
Differentially expressed (>3-fold) proteins of spermatozoa at different steps of cryopreservation
| Spot no. | Protein | NCBI no. | MASCOT scorea | Expression ratiob | Search |
|---|---|---|---|---|---|
| 228 | Major fibrous sheath protein chain D, bovine mitochondrial | gi|4588120 | 88.0 | 3.87 | NCBI |
| 1304 | F1-ATPase complexed with aurovertin B | gi|1827812 | 183.0 | 12.57 | NCBI |
| 2311 | PREDICTED: Uncharacterized protein LOC616410 | gi|119914715 | 299.0 | 0.25 | NCBI |
| 4208 | Triosephosphate isomerase | gi|61888856 | 174.0 | 6.48 | NCBI |
| 4418 | Nucleoside diphosphate kinase 7 | gi|62751773 | 64.0 | 6.13 | SwissProt |
| 4607 | Outer dense fiber protein 2 | gi|84000345 | 290.0 | 0.46 | NCBI |
| 5204 | NADH dehydrogenase flavoprotein 2 | gi|72004 | 67.0 | 5.30 | SwissProt |
| 5305 | F-actin-capping protein subunit beta | gi|28603770 | 53.0 | 5.73 | NCBI |
| 7206 | Superoxide dismutase, mitochondrial | gi|88853816 | 74.0 | 3.92 | NCBI |
aMASCOT scores are −10×log(P), where P is the probability that the observed match is a random event. Individual scores > 40 indicate identity or extensive homology (P < 0.05)
bExpression ratio is the ratio of relative volume of protein spots with after cryopreservation value to over before