Fig. 3.

Sequential G-banding and metaphase FISH was performed to refine initial karyotyping result. a G-banded metaphase. b Metaphase FISH indicated ETV6 (green) and RUNX1 (red) fusion as well as RUNX1 amplification. c No deletions for D20S108/20q12 probe in red, one signal on a normal chromosome 20 and the other signal on the derivative chromosome 3. d No deletions for D5S23/D5S721(5p15.2) in green and EGR1 (5q31) in red). e No rearrangement for CSF1R/ 5q33–34, however, one copy was translocated to chromosome 22. f Homozygous deletion of CDKN2A (9p21) in red; centromere 9 in green. g Hemizygous deletion of TP53 (17p13.1) in red; centromeric 17 in green. h Whole chromosome painting (WCP) for 17 (green) stained three different chromosomes, indicated translocations. i WCP for 22 (green) stained three different chromosomes, indicated translocations