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. 2016 Nov 21;13:81. doi: 10.1186/s12977-016-0312-7

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© The Author(s) 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 6 — N197D mutation in JRCSF restores CD4-bs-directed bNAb binding to JRCSF. a FACS-based cell surface staining assay of JRFL, JRCSF and the mutants C2, N197D, N230D, N289K (all in JRCSF) with 2G12 and b12 over a range of antibody concentrations. b FACS-based cell surface assay of JRFL, JRCSF and the mutant JRCSF (N197D) with VRC01 over a range of antibody concentrations