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. 2016 Nov 21;13:81. doi: 10.1186/s12977-016-0312-7

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© The Author(s) 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 9 — Cleavage property of JRCSF Env. a Cell surface staining assay of JRCSF and JRCSFSEKS with PG9, PGT151 and PGT145 over a range of antibody concentrations. b ELISA assay of gp120 shedding of JRCSF transfected 293T cells incubated with and without 50 μg/ml sCD4-183. c Western blot analysis of VRC01 immunoprecipitates of plasma membrane fraction of JRFL and JRCSF(N197D) transfected 293T cell lysates with anti-clade B Env rabbit Abs. d Western blot analysis of VRC01 immunoprecipitate of plasma membrane fraction of JRCSF(N197D) transfected 293T cell lysates and crude lysate of YU2 with anti-clade B Env rabbit Abs