Table 3.
Digestive enzymes expressed in Olavius algarvensis
| Transcript ID a) | Protein ID | Annotation | Consensus localization evidenceb) | Substrate or function |
|---|---|---|---|---|
| Protein digestion | ||||
| comp310626_c3 | n.d. | pancreatic carboxypeptidase A1 | secreted | release of C-terminal amino acids |
| comp330196_c1 | n.d. | pancreatic carboxypeptidase A2 | secreted | release of C-terminal amino acids |
| comp209868_c0 | n.d. | pancreatic carboxypeptidase A2 | secreted | release of C-terminal amino acids |
| comp329532_c3 | n.d. | pancreatic carboxypeptidase A2 | secreted or membrane | release of C-terminal amino acids |
| comp328734_c12 | n.d. | carboxypeptidase | secreted | peptides |
| comp328734_c1 | n.d. | carboxypeptidase | secreted | peptides |
| comp328734_c4 | n.d. | carboxypeptidase | secreted | peptides |
| comp326419_c0 | n.d. | uncharacterized carboxypeptidase | secreted | peptides |
| comp330196_c2 | n.d. | uncharacterized carboxypeptidase | secreted | peptides |
| comp319717_c3 | n.d. | lysosomal Pro-X carboxypeptidase | lysosomal | proline - amino acid bonds |
| comp320275_c1 | n.d. | chymotrypsin A | secreted | Tyr-/Trp-/Phe-/Leu-|-Xaa bonds |
| comp306409_c1 | n.d. | chymotrypsin B | secreted | Tyr-/Trp-/Phe-/Leu-|-Xaa bonds |
| comp334148_c2 | BF11_334148_c2_seq1_11 BF11_334148_c2_seq2_10 | chymotrypsin-like protease ctrl-1 | secreted | proteins |
| comp331491_c1 | BF11_331491_c1_seq1_7 | pancreatic elastase | secreted | proteins |
| comp334775_c2 | n.d. | cathepsin B | secreted | Arg – Arg/–Xaa bonds |
| comp335560_c0 | BF11_335560_c0_seq5_5 BF11_335560_c0_seq9_5 | cathepsin B | lysosomal | Arg – Arg/–Xaa bonds |
| comp306522_c2 | n.d. | cathepsin F | lysosomal | peptides, cleaves Phe/Leu |
| comp306522_c3 | n.d. | cathepsin F | lysosomal | peptides, cleaves Phe/Leu |
| comp308536_c0 | BF11_308536_c0_seq1_12 | cathepsin F | lysosomal | peptides, cleaves Phe/Leu |
| comp283346_c1 | n.d. | cathepsin L | lysosomal | proteins |
| comp306922_c1 | BF11_306922_c1_seq1_34 | cathepsin L | lysosomal | proteins |
| comp315575_c0 | n.d. | cathepsin L | lysosomal | proteins |
| comp315575_c2 | FD_315575_c2_seq1:64:270:1:+ | cathepsin L | lysosomal | proteins |
| comp328653_c0 | n.d. | cathepsin L | lysosomal | proteins |
| comp329800_c6 | n.d. | cathepsin O | lysosomal | peptides (endopeptidase) |
| comp314408_c1 | n.d. | cathepsin Z | lysosomal | C-terminal amino acids (not Pro) |
| comp308700_c0 | n.d. | cathepsin C (dipeptidyl peptidase I) | lysosomal | release of an N-terminal dipeptide |
| comp329456_c1 | n.d. | cathepsin C (dipeptidyl peptidase I) | lysosomal | release of an N-terminal dipeptide |
| comp328746_c4 | n.d. | alpha amylase | secreted | α1- > 4 glycosidic bonds |
| comp324906_c1 | BF11_324906_c1_seq1_9 BF11_324906_c1_seq2_12 | alpha amylase | secreted or membrane | α1- > 4 glycosidic bonds |
| comp335205_c1 | BF11_335205_c1_seq1_20 | lysosomal alpha glucosidase | secreted or membrane | α1- > 4 glycosidic bonds |
| comp320084_c0 | n.d. | lysosomal beta-mannosidase | lysosomal | cleaves terminal β-D-mannose |
| comp334411_c3 | n.d. | sucrase-isomaltase | secreted or membrane | α1- > 6 glycosidic bonds |
| comp329957_c8 | BF11_329957_c8_seq2_17 | acid trehalase | secreted or membrane | trehalose - > glucose |
| comp335402_c7 | n.d. | acid trehalase | secreted or membrane | trehalose - > glucose |
| Lipid degradation | ||||
| comp22535_c0 | n.d. | lysosomal acid lipase | secreted | hydrolyzes steryl esters |
| comp249291_c0 | n.d. | lysozyme | secreted | peptidoglycan (glycosidic bonds) |
| comp250229_c0 | BF11_250229_c0_seq1_15 BF11_250229_c0_seq2_17 | peptidoglycan recognition protein | secreted | peptidoglycan (peptide bonds) |
| comp335695_c10 | n.d. | peptidoglycan recognition protein | secreted or membrane | peptidoglycan (peptide bonds) |
| comp330541_c4 | n.d. | peptidoglycan recognition protein | secreted | peptidoglycan (peptide bonds) |
| comp314994_c0 | n.d. | peptidoglycan recognition protein | secreted or membrane | peptidoglycan (peptide bonds) |
| comp332570_c2 | n.d. | peptidoglycan recognition protein | secreted or membrane | peptidoglycan (peptide bonds) |
a)Defined as Trinity components; see [13]
b)Probable subcellular localization of proteins based on the results of TMHMM [117, 118], SignalP-4 [119], Phobius [120], TargetP [121], DISTILL [122], LocTree3 [123], BaCelLo [124], and iLoc-Animal [125]. Secreted – enters secretory pathway and is either excreted to the extracellular space or confined to non-cytoplasmic insides of intracellular compartments; membrane – predicted to be membrane integral; lysosomal – predicted to be targeted towards the lysosome. See Additional file 3: Table S14 for details on localization evidence