. 2016 Nov 21;9:252. doi: 10.1186/s13068-016-0665-0

Table 2.

The macromolecular composition of T. reesei

Biomass component			% (w/w)
Proteins			45.100 ^a
Ala			2.715^b
Arg			3.944^b
Asn			1.613^b
Asp			2.677^b
Cys			0.549^b
Gln			2.063^b
Glu			3.200^b
Gly			1.649^b
His			1.346^b
Ile			2.186^b
Leu			4.101^b
Lys			2.595^b
Met			1.202^b
Phe			2.220^b
Pro			2.363^b
Ser			2.808^b
Thr			2.354^b
Trp			1.144^b
Tyr			1.846^b
Val			2.523^b
Carbohydrates			23.190 ^a
Chitin			7.820^c
Other carbohydrates			15.370^d
RNA			6.122 ^a ^b
DNA			0.912 ^a
Lipids			4.176 ^g
Fatty acids-esters
Myristic acid (C14:0) est			0.004^a
Palmitic acid (C16:0) est			0.613^a
Palmitoleic acid (C16:1n-7) est			0.011^a
Stearic acid (C18:0) est			0.070^a
Oleic acid (C18:1n-9) est			0.126^a
Linoleic acid (C18:2n-6) est			1.425^a
$α$ -linolenic acid (C18:3n-3) est			0.292^a
Arachidic acid (C20:0) est			0.004^a
Lignoceric acid (C24:0) est			0.005^a
Fatty acids-free
Palmitic acid (C16:0) FFA			0.060^a
Stearic acid (C18:0) FFA			0.012^a
Oleic acid (C18:1n-9) FFA			0.107^a
Linoleic acid (C18:2n-6) FFA			0.274^a
Ergosterol			0.278^a
Triacylglycerol			1.792^e
Phosphatidylethanolamine			0.551^e
Phosphatidylcholine			1.102^e
Ash			5.100 ^f

^aMeasured as described in "Methods" section

^b Amino acid ratios calculated based on codon ratios in RNAseq transcriptome data, with transcripts encoding secreted proteins removed

^c Estimated based on the chitin content (% w/w) of A. oryzae biomass [8], and corrected for different ash contents in A. oryzae and T. reesei preparates

^d Carbohydrates other than chitin

^e Calculated based on the measured ratio of TAG:PE:PC 52:16:32 and the measured amount of fatty acid esters

^f Measured from lactose-limited chemostat cultures of T. reesei Rut-C30 (D = 0.051/h) described in [40]

^g Sum of measured free fatty acids, ergosterol, triacylglycerol, phosphatidylethanolamine and phosphatidylcholine