Table 3. Phenotypic changes in different genotypes of rapid-cycling B. rapa plants after 5-azaC treatment.
Genotype A | Genotype B | Genotype C | Genotype D | Genotype E | ||||||
---|---|---|---|---|---|---|---|---|---|---|
Plant trait | change | P value | change | P value | change | P value | change | P value | change | P value |
Plant Height [cm] | ↓ | < 0.001 | ↓ | < 0.001 | ↓ | < 0.001 | - | 0.310 | ↓ | 0.001 |
Time to Flowering [d] | ↑ | < 0.001 | - | 0.109 | ↑ | < 0.001 | - | 0.373 | - | 0.017 |
Leaf Number | ↓ | < 0.001 | ↓ | 0.022 | ↓ | < 0.001 | - | 0.597 | - | 0.158 |
Bud Number | ↓ | < 0.001 | ↓ | < 0.001 | ↓ | < 0.001 | - | 0.705 | ↓ | 0.004 |
Flower Number | ↓ | 0.013 | - | 0.140 | - | 0.107 | - | 0.140 | ↓ | 0.022 |
Flower Petal Area [mm2] | ↓ | < 0.001 | ↓ | < 0.001 | ↓ | < 0.001 | ↓ | 0.022 | ↓ | 0.022 |
Plant Petal Area [mm2] | ↓ | < 0.001 | ↓ | < 0.001 | ↓ | < 0.001 | - | 0.338 | ↓ | 0.002 |
Nectar Amount [μl] | ↓ | < 0.001 | - | 0.948 | ↓ | 0.019 | - | 0.948 | - | 0.058 |
Phenylethyl Alcohol | - | 1.000 | - | 0.196 | ↓ | 0.001 | - | 1.000 | - | 1.000 |
Multiple comparisons show a decreased phenotypic performance in response to genome demethylation in all five different plant families (genotype A-E). However, the five genotypes differ in their susceptibility to the 5-azaC-treatment. While some genotypes (e.g. genotype C) exhibit phenotypic changes in a large number of traits, other genotypes such as genotype D are almost unaffected. Arrow up: trait increase, arrow down: trait decrease, dash: no trait change, P value of significant changes in bold (α = 0.05).