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. 2016 Nov 21;11(11):e0166730. doi: 10.1371/journal.pone.0166730

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© 2016 Mondal et al

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig 5 — (A) Graphical presentation of growth inactivation assay using K. pneumoniae strain with different antimicrobial compounds and efflux pump inhibitors is shown by OD_600nm at every 15 minutes time interval. Growth inactivation assays (GIAs) were done with a representative strain to know the impact on drug efflux capability using substrates KAN: kanamycin, ACRI: acriflavine and BENZ: benzalkonium chloride, in presence and absence of efflux pump inhibitors (CCCP: carbonyl cyanide 3- chlorophenylhydrazone, DNP: 2, 4 -dinitrophenol, VER: verapamil, and RES: reserpine). The graph represents the mean of three independent experiments. (B) Protein gel picture of OMP profile of representative K. pneumoniae strains. OMP was isolated from the strains using sarkosyl method and was checked on SDS-PAGE. K. pneumoniae isolates showed differential expression in our collection.