Fig. 3.

Characterisation and validation of humanised RadA mutants. (a) Surface representation of selected humanised RadA mutants with surfaces coloured as in Fig. 1B, with humanising mutations coloured in dark green. HumRadA33 as the final mutant is shown for completeness, but in the absence of its crystal structure, the humanisation is shown on the structure of HumRadA22. (b) Superposition of the HumRadA22 and human RAD51 in the FxxA (left) and LFDE (right) sites with each RadA residue coloured in green if it is identical to human residue. All RAD51 residues are shown in light grey, with the key residues discussed in the text labelled following RadA residue numbering.