Fig 2. Bacterial growth and biofilm formation by H. pylori 26695 wild-type, cagA and cagPAI deletion mutants.

(A) Bacterial growth measured at OD600nm, (B) biofilm formation analyzed using crystal violet analysis and (C) specific biofilm unit. Specific biofilm unit is a representation of biofilm formation normalized with planktonic mass and is determined by dividing the absorbance of biofilm-bound crystal violet with the corresponding planktonic cell turbidity OD. Biofilm development of different H. pylori strains was monitored over a period of 14 days. Values and error bars represent means and standard deviations of measurements in triplicates. (*) p< 0.05 (2-tailed Student’s t test).