Figure 3.
Tripartite motif-containing protein 16 (TRIM16) protein and mRNA expression is decreased in distant metastatic tissues (n=6) compared with that in non-distant metastatic tissues (n=7). (A) TRIM16 protein levels in distant metastatic tissues (n=6) and non-distant metastatic tissues (n=7) were measured by western blot analysis. (B) Analysis of TRIM16 protein expression in distant metastatic tissues and non-distant metastatic tissues. (C) TRIM16 expression was measured in distant metastatic tissues and non-distant metastatic tissues by qPCR. (D) TRIM16 protein levels in prostate cancer cell lines (PC3, Du145, and LNCaP) and a non-cancerous prostate epithelial cell line (RWPE-1) were measured by western blot analysis. **P<0.01 is based on the Student's t-test. All results are from three independent experiments. Error bars indicate the means ± SD. Met, metastases.