Table 2. Effect of S deficiency on relative expression of genes: AtSultr1.1, AtSultr2.1, AtSDI1 and AtAPR3 in roots of sultr1.1 and sultr1.2 mutants of Arabidopsis thaliana expressed relatively to wildtype Col-0 grown under sufficient S supply (Control).
| Arabidopsis mutants | ||||||
|---|---|---|---|---|---|---|
| Col-0 | sultr1.1 KO | sultr1.2 (sel1-8) | ||||
| Gene expression | Control | Low S | Control | Low S | Control | Low S |
| AtSultr1.1 | 1.0±0.20a | 15.5±4.99b | 0c | 0c | 13.8±0.23b | 74.4±9.40d |
| AtSultr1.2 | 1.0±0.12a | 3.2±0.61b | 1.0±0.07a | 1.8±1.06a | 3.4±0.52b | 4.4±0.53b |
| AtSDI1 | 1.0±0.13a | 41.9±14.31b | 1.5±0.31a | 81.6±37.03b | 19.0±2.40b | 167.2±16.59c |
| AtAPR3 | 1.0±0.14a | 3.6±0.53b | 1.1±0.08a | 4.3±0.19b | 2.7±0.09a | 5.0±0.11c |
| Leaf concentration | ||||||
| S (mg.g-1 DW) | 11.8±0.44a | 5.3±0.06b | 12.7±0.27a | 5.6±0.03b | 6.9±0.09d | 5.0±0.11b |
| Mo (ug.g-1 DW) | 28.7±1.22a | 192.7±10.49b | 31.9±0.55a | 170.0±10.49b | 16.3±0.33c | 134.9±5.07d |
| [Mo]:[S].104 | 24.2±0.12a | 365±5.7b | 25.14±0.73a | 304±18.0c | 23.7±0.44a | 270±7.4c |
In the sultr1.2 mutant the transcript was present, because the gene is inactivated by a point mutation in coding sequence [38]. Leaf concentrations of S and Mo and the [Mo] to [S] ratios (i.e. [Mo]:[S] multiplied by 104 for easier reading) are also given for the same plants. Values from the same line (i.e. between genotypes and S treatments for a given transcript or a nutrient concentration) are significantly different at P<0.05 when marked by different letters. Data are given as the mean ± SE (n = 3 for PCR analysis, n = 4 for S and Mo analysis).