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. 2016 Nov 8;38(6):1727–1733. doi: 10.3892/ijmm.2016.2798

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Copyright: © Liu et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Overexpression of DEC1 or DEC2 has no effect on the expression of apoptotic markers in DU145 and PC-3 cells. DU145 and PC-3 cells were transfected with DEC1 pcDNA (A and B) or DEC2 pcDNA (C and D) and incubated for 24 h. The cells were treated with paclitaxel (50 μM) and incubated for an additional 24 h, before being lysed. The lysates were subjected to western blot analysis of DEC1, DEC2, cleaved PARP, cleaved caspase-8, Bcl-2 and actin. One representative of at least three independent experiments with similar results is shown. DEC, differentiated embryonic chondrocyte; PARP, poly (ADP-ribose) polymerase.