Fig 5. Effects of picroside II on mRNA expression of inflammatory cytokines/mediators in lung tissues.

The mRNA levels of (A) Th2-related cytokines, (B) Th1-related cytokine, (C) Th17-related cytokine, and (D) asthma-related mediators, were determined by real-time RT-PCR. The data were normalized to Gapdh gene expression. NC; normal control mice treated with saline only, model; HDM-sensitized/challenged mice, PIC II; picroside II (30㎎/㎏) + HDM-sensitized/challenged mice, DEX; dexamethasone + HDM-sensitized/challenged mice. All data are representative of three independent experiments and represented as the mean ± SEM (n = 6mice/group). ^##p<0.01 and ^###p<0.001, compared with normal control (NC); *p<0.05, **p<0.01, and ***p<0.001, compared with model group.