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. 2016 Aug 25;115(9):1058–1068. doi: 10.1038/bjc.2016.259

Figure 1.

Figure 1

Representative phase-contrast micrographs of STS primary cell lines.(A) Shef-UPS 01-spindle-shaped cells at passage 69 without distinct colony formation in culture. (B) Shef-UPS 03 cultures at passage 35 showing homogeneous cultures of long spindle-shaped cells growing in loose colonies. (C) Shef-DDLPS 01 cultures at passage 71 showing pleomorphic cells without distinct colony formation. (D) Shef-DDLPS 02 cultures at passage 22 composed of a combination of spindle-shaped cells (white arrows) and round, histiocyte-like cells (black arrows), both growing in distinct colonies. (E, F) Cells derived from Shef-LMS 01 growing in separate cultures designated as Ws (passage 69) and w1 (passage 56), respectively. (G, H) Morphologically distinct cells derived from Shef-MFS 01 growing in separate adherent cultures designated as w1 (passage 35) and w2 (passage 31), respectively. Scale bars=100 μm. DDLPS=dedifferentiated liposarcoma; LMS=leiomyosarcoma; MFS=myxofibrosarcoma.