Table 2. Characteristics of primary soft tissue sarcoma cell lines and their parent tumours.

Cell line	Laboratory designation	Morphology	Doubling timea (h)	Age (years)/gender	STS subtype	Site	Size	TNM stage	Immunocytochemistry
Shef-UPS 01	STS 14/10	Spindle-shaped cells with distinct nuclei No pleomorphism No distinct colony formation	p70=40.55	53/F	Undifferentiated pleomorphic sarcoma	Lower limb	230 mm	pT2b Stage III	Vimentin – positive CK, SMA, c-Kit – negative
Shef-UPS 02	STS 06/11	Pleomorphic mostly spindle-shaped cells No distinct colony formation	p91=35.38	76/M	Undifferentiated pleomorphic sarcoma	Lower limb	170 mm	pT2b Stage III	Vimentin – positive CK, SMA, c-Kit – negative
Shef-UPS 03	STS 09/11	Long spindle-shaped cells No pleomorphism Loose colony formation	p35=63.97	66/F	Undifferentiated pleomorphic sarcoma	Lower limb	115 mm	pT2b Stage III	Vimentin – positive CK, SMA, c-Kit – negative
Shef-DDLPS 01	STS 09/10	Mostly spindle-shaped cells Some pleomorphism No distinct colony formation	w2p35=49.5	68/F	Dedifferentiated liposarcoma	Retroperitoneum	300 mm	pT2b Stage III	Vimentin – positive CK, SMA, c-Kit – negative
bShef-DDLPS 02	STS 20/11	Two distinct cell types in same culture (A) long spindle-shaped cells (B) Rounded, histiocyte-like cells with distinct nuclei Both form tight colonies	p23=58.22	70/F	Dedifferentiated liposarcoma	Lower limb	170 mm	ypT2b Stage III	Vimentin, SMA – positive CK, c-Kit – negative
Shef-LMS 01	STS 02/11
	w1	Long spindle-shaped cells No pleomorphism Tight colony formation	w1p54=27.44	62/F	Leiomyosarcoma	Pelvis	135 mm	pT1b Stage IIA	Vimentin, SMA – positive CK, c-Kit – negative
	ws	Rounded, histiocyte-like cells with distinct nuclei No pleomorphism Tight colony formation	wsp63=44.62
Shef-MFS 01	STS 21/11
	w1	Rounded, histiocyte-like cells No pleomorphism Tight colony formation	w1p35=59.93	73/M	Myxofibrosarcoma	Upper limb	50 mm	pT1b Stage IIA	Vimentin – positive CK, SMA, c-Kit – negative
	w2	Polygonal cells No pleomorphism Loose colony formation	w2p31=56.30

Abbreviations: CK=cytokeratin; c-Kit=CD117; DDLPS=dedifferentiated liposarcoma; LMS=leiomyosarcoma; MFS=myxofibrosarcoma; SMA=smooth muscle actin; UPS=undifferentiated pleomorphic sarcoma.

^aPassage number at which proliferation assay was performed is indicated.

^bPatient received neoadjuvant radiotherapy.