Table 3. STR profiles of primary STS cultures.
Cell line/passage | THO1 | D21S11 | D5S818 | D13S317 | D7S820 | D16S539 | CSFIPO | AMEL | vWA | TPOX |
---|---|---|---|---|---|---|---|---|---|---|
aShef-UPS 01 | ||||||||||
p31 | 6,7 | 27,30 | 12,13 | 8,11 | 8 | 14 | 12 | X | 16 | 8 |
p68 | 6,7 | 27,30 | 12,13 | 8,11 | 8 | 13,14 | 12 | X | 16 | 8 |
aShef-UPS 02 | ||||||||||
p41 | 6,9.3 | 29,31 | 9,13 | 14 | 8,11 | 11 | 10,11 | X, Y | 17,18 | 8 |
p83 | 6,9.3 | 29,31 | 9,13 | 14 | 8,11 | 11 | 10,11 | X | 17,18 | 8 |
Shef-UPS 03 | ||||||||||
p34 | 9.3 | 28,31.2 | 9,12 | 11 | 10 | 9 | 10 | X | 17 | 8 |
aShef-DDLPS 01 | ||||||||||
w2p35 | 6 | 29,32 | 12,13 | 8,14 | 9,10 | 9,10 | 10,14 | X | 17,18 | 11 |
w2p70 | 6 | 29,32 | 12,13 | 8,14 | 9, 10 | 9,10 | 10,14 | X | 17,18 | 11 |
Shef-DDLPS 02 | ||||||||||
p2 | 9 | 29 | 9 | 14 | 10,12 | 12 | 12 | X | 20 | 9,11 |
aShef-LMS 01 | ||||||||||
w1p16 | 9 | 27,30 | 11 | 14 | 10,11 | 11 | 10,12 | X | 16 | 8 |
w1p54 | 9 | 27,30 | 11 | 14 | 10,11 | 11 | 10 | X | 16 | 8 |
wsp27 | 9 | 27,30 | 11 | 14 | 10,11 | 11 | 10,12 | X | 16 | 8 |
wsp63 | 9 | 27,30 | 11 | 14 | 10,11 | 11 | 10,12 | X | 16 | 8 |
Shef-MFS 01 | ||||||||||
w1p35 | 8 | 30,31.2 | 12,13 | 13 | 8,9 | 12 | 10 | X, Y | 14,16 | 11 |
w2p31 | 8 | 30,31,2 | 12,13 | 13 | 8,9 | 12 | 10 | X, Y | 14,16 | 11 |
Abbreviations: DDLPS=dedifferentiated liposarcoma; LMS=leiomyosarcoma; MFS=myxofibrosarcoma; STR=short tandem repeat; STS=soft-tissue sarcoma; UPS=undifferentiated pleomorphic sarcoma.
Profiles comprise the alleles at 10 STR loci. No significant match (>80% relatedness) was found when compared with profiles in the COGcell database (http://strdb.cogcell.org/).
Profiles for morphologically distinct variants derived from two STS cases (shown in bold) are identical
STR profiling repeated after 1 year (30–40 subsequent passages) and disparities in allele matching is in italics.