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. Author manuscript; available in PMC: 2017 Mar 26.
Published in final edited form as: Nat Chem Biol. 2016 Sep 26;12(11):973–979. doi: 10.1038/nchembio.2200

Figure 1. Biosynthesis of microviridin J.

Figure 1

(a) The microviridin J biosynthetic pathway in Microcystis aeruginosa MRC contains three gene products: MdnC and MdnB catalyze the macrocyclization of MdnA to generate the intermediates MdnAΔ2 and MdnAΔ3, respectively. The cleavage of leader peptide followed by an acetylation produces the mature microviridin J. The residues coupled by MdnC are colored red and likewise MdnB, blue. (b) In vitro cyclization of MdnA with purified MdnC and MdnB enzymes. HPLC analysis shows separation of the precursor peptide and two macrocyclization intermediates (wavelength=220 nm).