Figure 4. MdnC uses ATP to catalyze the macrocyclization of the precursor peptide MdnA.
(a) Modelled ADP interacts with the C-domain and central domain of MdnC. The positions of Mg2+ and ADP are adapted from the aligned LysX structure (PDB entry 3VPD, 20% sequence identity). (b) Interactions of MdnC with the precursor peptide MdnA. The strictly conserved region of MdnA (PFFARFL) is well-resolved in the electron density map. Helix α7 and β9β10 hairpin of MdnC sandwich the helical region of the resolved MdnA leader peptide. Calculated mFo-DFc map is displayed at a 1.5σ contour level.