Table 1. Results of crossing experiments to establish linkage group of the transgene insertion in the C42 strain.

Cross	Male				Female
	EGFP(+)		EGFP(−)		EGFP(+)		EGFP(−)
	Black	Red	Black	Red	Black	Red	Black	Red
RED ♀ X C42 ♂	652	—	3a	—	1a	—	695	—
RED ♀ X F1 (RED ♀ X C42 ♂) ♂	878	20b	—	3a	2a	—	25b	787

Abbreviations: EGFP, enhanced green fluorescent protein; +, EGFP positive; −, EGFP negative.

Figures represent numbers of progeny individuals with a given phenotype. Mosquitoes were scored at the pupal stage for sex, EGFP expression and eye color. The RED strain individuals are homozygous for a recessive red eye color mutation. Black denotes a wild-type eye phenotype.

^aIndividuals carrying a recombinant chromosome 1 with the breakpoint between the sex locus and the transgene.

^bIndividuals carrying a recombinant chromosome 1 with the breakpoint between the sex locus and the re locus.