Figure 4.

rn-Gal4 RNAi knockdown of plausible candidate genes implicated by QTL. Each bar represents the mean tooth number on a single comb (±1 s.d.) for several rn-Gal4/UAS male RNAi knockdown (light gray bars) and control genotypes (dark gray bars). The name of each UAS control or gene is provided beneath each bar along with the stock number of the UAS strain in the Bloomington Drosophila Stock Center (TRiP) or the Vienna Drosophila Resource Center (VDRC). The sample size per genotype is presented at the bottom of each bar. The phenotype of each knockdown was compared with the appropriate control via Welch's t-tests (NS, not significant, *P<0.01, **P<0.0005, ***P<10^–10). For TRiP lines we tested three control strains—‘Luc' (UAS-Luciferase), ‘LS' (landing site for phiC31-based transgenesis) and ‘GFP' (UAS-GFP)—and here present statistical comparison of each gene knockdown to ‘Luc'. The ‘Luc' control genotype was tested in all experimental blocks, and no significant block-to-block variation was observed for this genotype. For VDRC-GD and -KK lines the control genotype is listed as ‘Con'.