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. 2016 Oct 24;5(10):e263. doi: 10.1038/oncsis.2016.63

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Copyright © 2016 The Author(s)

Oncogenesis is an open-access journal published by Nature Publishing Group. This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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Forskolin rescues effects of GPR133 knockdown. (a) The GPR133-KD no. 1-induced impairment of tumorsphere formation in hypoxia is rescued by Forskolin treatment (10 μM) in GBML8 (i) (n=3 experiments, two-way ANOVA F_(3,6)=7.726, P<0.02) and GBML20 (ii) (n=3 experiments, two-way ANOVA F_(3,6)=9.655, P<0.01). Multiple comparisons were performed with Tukey's post hoc tests. (b) Ten micromolar Forskolin treatment increases cAMP levels in GBML8 (i) (n=3 experiments, t-test, P<0.0006) and GBML20 (ii) (n=3 experiments, t-test, P<0.009) under hypoxic conditions. (c) GPR133 knockdown decreases cAMP levels in GBML8 and GBML20 under normoxic conditions (n=3 experiments per condition, ANOVA F_(1,8)=17.96, P<0.003).