Fig. 2.

Characterization of jasmonic inducible pathogenesis-related class 10 (JIOsPR10) overexpression plants. (A) Schematic diagram of JIOsPR10 under rice cytochrome c promoter with basta selection marker was used for transformation. RB, right border; OsCc1 pro, rice cytochrome c promoter; TpinII, pinII terminator; BAR, basta resistance marker; Tnos, nos terminator; LB, left border. (B) Genotyping of overexpression plants by PCR with Basta selection marker specific primers. M, DNA marker; EV, empty vector plasmid set as positive control; WT; wild type. (C) Southern blot analysis of JIOsPR10 overexpression plants with BAR probe. (D) RT-PCR analysis of JIOsPR10 expression in WT and overexpression plants. Number indicate the relative expression level of JIOsPR10 compare to actin2. (E) Western blot analysis of JIOsPR10 in WT and overexpression plants with JIOsPR10 specific antibody. Equal loading of protein samples was detected by PonceauS staining.