Table 1.
Identification of differentially modulated protein spots in wild type and JIOsPR10 overexpression plants using MALDI-TOF mass spectrometer
| Spot No. | Protein name | Accession No. | Score | Database | MP | SC (%) | Ex Mr (kDa) | Th Mr (kDa) | Ex pI | Th pI | Biological function |
|---|---|---|---|---|---|---|---|---|---|---|---|
| 1 | Thaumatin-like protein | ACA50508 | 479 | Prospector | 3 | 21.0 | 18.0 | 18.1 | 4.4 | 6.5 | Defense |
| 2 | DUF26-like protein | AAW34053 | 475 | Prospector | 6 | 38.4 | 24.4 | 27.3 | 4.7 | 5.0 | Defense |
| 3 | Beta-1,3-glucanase | BAA77783 | 422 | Prospector | 4 | 15.9 | 32.2 | 34.2 | 4.6 | 4.6 | Carbohydrate metabolic process |
| 4 | Peroxidase | CAA46916 | 86 | Mascot | 7 | 29.0 | 37.1 | 32.9 | 5.2 | 5.8 | Response to oxidative stress |
| 5 | Enolase | ABB46862 | 374 | Prospector | 13 | 32.0 | 48.0 | 48.0 | 5.5 | 5.4 | Glycolytic process |
| 6 | Nucleoside diphosphate kinase | AAT08712 | 396 | Prospector | 4 | 27.0 | 18.5 | 17.7 | 5.4 | 9.1 | Energy |
| 7 | Formate dehydrogenase | NP_001057666 | 112 | Mascot | 8 | 32.0 | 36.6 | 41.2 | 6.4 | 6.9 | Response to oxidative stress |
| 8 | Cu/Zn-superoxide dismutase | BAB21760 | 228 | Prospector | 7 | 42.0 | 41.4 | 21.3 | 6.6 | 5.8 | Response to oxidative stress |
| 9 | Xylanase inhibitor protein 1 | NP_001057599 | 65 | Mascot | 5 | 24.0 | 44.6 | 27.4 | 6.9 | 6.4 | Carbohydrate metabolic process |
| 10 | Cytoplasmic malate dehydrogenase | AAG13573 | 372 | Prospector | 8 | 21.0 | 32.9 | 35.6 | 6.5 | 5.7 | Tricarboxylic acid cycle |
JIOsPR10, jasmonic inducible pathogenesis-related class 10; MP, matched peptides; SC, sequence coverage; Ex Mr, experimental molecular mass; Th Mr, theoretical molecular mass; Ex pI, experimental isoelectric point; Th pI, theoretical isoelectric point.