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. 2016 Nov 15;10:3755–3761. doi: 10.2147/DDDT.S115121

Figure 5.

Figure 5

ZD55-IL-18 and DTIC demonstrated synergistic effects to induce apoptosis and upregulate VEGF expression in melanoma mouse xenografts.

Notes: (A) ZD55-IL-18 plus DTIC exhibited synergistic effects in the induction of apoptosis in melanoma A375 mouse xenografts. Tumor sections were excised and analyzed for apoptosis by TUNEL staining. Cells stained with brown granule in the nucleus were judged as apoptotic cells. (B) Immunohistochemical analysis of VEGF expression in melanoma A375 mouse xenografts is shown. (C) The lung and liver tissues were excised from nude mice in different treatment groups and analyzed by HE staining. Magnification ×400. Scale bar 5 µm. *P<0.05, **P<0.01.

Abbreviations: DTIC, dacarbazine; VEGF, vascular endothelial growth factor; TUNEL, terminal deoxynucleotidyl transferase dUTP nick end labeling; dUTP, 2′-deoxyuridine-5′-triphosphate; HE, hematoxylin and eosin; PBS, phosphate-buffered saline; IL-18, interleukin-18.