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. Author manuscript; available in PMC: 2016 Nov 21.
Published in final edited form as: ACS Chem Biol. 2016 Mar 4;11(5):1354–1361. doi: 10.1021/acschembio.5b00934

Table 1. EC50, pEC50, and Efficacy Values for 1 and the Top Six HssRS Activators from the Single Point Screena.

Cmpd Structure EC50(μM) pEC50 Efficacy (%)
1 graphic file with name nihms829264t1.jpg 11.6 4.90 ± 0.37 100
la graphic file with name nihms829264t2.jpg 30.4 4.49 ± 0.083 28.2 ± 5.4
lb graphic file with name nihms829264t3.jpg 50.2 4.30 ± 0.20 26.2 ± 6.7
2a graphic file with name nihms829264t4.jpg ND ND 17.0 ± 2.9
2c graphic file with name nihms829264t5.jpg 14.6 4.66 ± 0.016 87.8 ± 23.7
2d graphic file with name nihms829264t6.jpg 5.81 5.24 ± 0.076 ND
2e graphic file with name nihms829264t7.jpg 10.7 4.97 ± 0.033 35.7 ± 10.1
2f graphic file with name nihms829264t8.jpg 13.6 4.90 ± 1.1 30.1 ± 8.0
a

EC50 and pEC50 were calculated from concentration response curves after 6 h of growth using the XylE reporter assay.

An EC50 for 2a could not be determined because the concentration response curve did not plateau below its solubility limit. Efficacy is the percent activation of HssRS compared to 1 at 50 μM (all compounds reach Emax at or below 50 μM except 2a). All data were collected in triplicate, and error values are one standard deviation from the mean.