Figure 6.

miR-29a inhibits the growth of NSCLC cells in nude mice.

Notes: (A) A549 cells were stably transfected with pYr-LVX-miR-29a lentiviral plasmid or pYr-LVX-scramble miR lentiviral plasmid as control. Real-time RT-PCR was conducted to examine the expression levels of miR-29a. (B) Nude mice were then subcutaneously implanted with these A549 cells. miR-29a overexpression in A549 cells effectively protected nude mice from tumor-induced death. (C) At 90 days after implantation, all mice that had not died were sacrificed. The tumor xenograft was obtained. (D) The weight and (E) volume of the tumor xenograft were examined. (F) Western blot assay was conducted to examine the protein expression of LASP1, Ki67, and MMP2 in xenografted tumor tissues. **P<0.01 and ***P<0.001 vs control.

Abbreviations: miR, microRNA; NSCLC, non-small cell lung cancer; RT-PCR, reverse transcription polymerase chain reaction.