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. Author manuscript; available in PMC: 2017 Nov 17.
Published in final edited form as: Cell Chem Biol. 2016 Oct 20;23(11):1351–1361. doi: 10.1016/j.chembiol.2016.09.012

Figure 5. ’882 Impairs Aconitase Function.

Figure 5

(A) Aconitase activity is reduced in NM and LAC cultured in the presence of ’882 after xylose induction of acnA transcription. *p < 0.001 by Student’s t test with Holm-Sidak correction for multiple comparisons, compared with induced + DMSO.

(B) Aconitase activity in LAC is inhibited by 50 μM ’882 to a greater extent as oxygen availability is reduced by decreasing the volume of gas above growth medium. *p < 0.05 relative to a headspace:culture volume of 10, calculated by one-way ANOVA with Sidak correction for multiple comparisons.

(C) The inhibition of aconitase by ’882 is greater under anaerobic conditions in LAC, where ~50% inhibition is achieved by 25 μM ’882, compared with 50 μM ’882 in (B). *p < 0.05 relative to no ’882 calculated by one-way ANOVA with Sidak correction for multiple comparisons.

(D) ’882 does not inhibit the activity of purified aconitase protein that has been chemically reconstituted with Fe-S cofactor in vitro prior to exposure to ’882.

For (A)–(D), error bars represent SD combined from two independent experiments. For (A)–(D), the acnA::Tn strain carried pacnA, which encodes for acnA under the transcriptional control of a xylose-inducible promoter. See also Figure S4.