(A) Aconitase activity is reduced in mutants lacking Fe-S cluster assembly factors when cultured in the presence of 50 μM ’882. *p < 0.05 by Student’s t test for each strain comparing DMSO with ’882.
(B) A LAC strain defective in Fe-S protein maturation is sensitive to 10 μM ’882 under microaerobic conditions in defined media lacking Ile and Leu. *p < 0.05 by Student’s t test for each time point comparing Δnfu ΔsufT + ‘882 with Δnfu ΔsufT + DMSO.
For (A) and (B), error bars represent SD combined from two independent experiments.