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Representative subcellular localization of WT and mutated ZIC3 proteins. (a–d) First column represent WT or mutant GFP-tagged ZIC3 proteins transfected in HeLa cells. Second column is the nuclear DAPI staining and the third column is both columns merged. A minimum of 50 cells were scored for each construct. Scoring was based on three localization classifications: nuclear, cytoplasmic or both (e).
