Figure 4.

Functional assessment of variants in zebrafish. (a) Representative micrographs of cmlc2:GFP zebrafish embryo's displaying cardiac laterality defects. The picture on the left shows a normally located left-sided heart (left-sided heart), while the picture in the middle indicates an intermediately positioned heart (midline heart). The picture on the right illustrates an aberrantly located right-sided heart (right-sided heart). (b) Quantification of phenotypes upon expression of wild-type human ZIC3 protein and of the indicated variants. A minimum of 80 embryos was analyzed. uic, uninjected control.