Table 2A. Breakdown of values of proliferation index according to tumour subtype and grade. There is an increase in proliferation index in clear cell renal cell carcinoma and papillary renal cell carcinoma according to grade. No such relationship is seen in the chromophobe renal tumours. Table 2B - There is remarkable correlation between quantitative and semiquantitative analysis for both markers. In group I the median value for Ki-67 is 4.05% in quantitative analysis while it is 5% in semiquantitative analysis. MCM-2 shows 10.96% in quantitative and 10.00 in semiquantitative analysis. For grade II Ki-67(quantitative) is 9.2% versus 5% in semi-quantitative. MCM-2 shows better correlation in this grade with median values of 16.21% versus 15%. In group III Ki-67(quantitative) is 12.7% and semi-quantitative is 12.5%. MCM-2 shows (quantitative)22.08% versus 20% in semi-quantitative analysis. Finally grade IV shows Ki-67(quantitative) 36.93% versus 40% in semiquantitative. MCM-2(quantitative) 50.99% versus 55% in semiquantitative. The mean values and standard deviations also correspond with each other. Note that Ki-67(semi-quantitative) median for grade I and II is same as compared to MCM-2. Spearman's Correlate is significant in all cases except for Ki-67 grade I.
| A) Correlation of grade with labelling index according to each subtype | B) Comparison of Labelling indices of Ki-67 and MCM-2 using quantitative and semi-quantitative analysis | ||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Clear cell Carcinoma | Papillary Renal cell carcinoma | Chromophobe renal cell carcinoma | Ki-67 (Quantitative) | Ki-67 (Semi-quantitative | Spearman's Correlation rank test(p) | MCM-2 (Quantitative) | MCM-2 (semi-quantitative) | Spearman's correlation rank test(p) | |||||
| Grade | Ki-67 | MCM-2 | Ki-67 | MCM2 | Ki-67 | MCM-2 | |||||||
| I | N | 6 | 6 | 1 | 1 | 1 | 1 | 8 | 8 | -0.051 | 8 | 8 | 0.884 |
| Median | 4.05% | 13.47% | 6.67% | 10.02% | 1.74% | 3.90% | 4.06% | 5.00% | p=0.904 | 10.97% | 10.00% | p=0.004 | |
| II | N | 8 | 8 | 2 | 2 | 2 | 2 | 12 | 12 | 0,88 | 12 | 12 | 0,876 |
| Median | 11,10% | 24,19% | 5,57% | 9,32% | 1,67% | 5,08% | 9,20% | 5,00% | p=0.000 | 16.22% | 15.00% | p=0.000 | |
| III | N | 9 | 9 | 2 | 2 | 3 | 3 | 14 | 14 | 0.726 | 14 | 14 | 0.756 |
| Median | 14.54% | 23.77% | 12.27% | 24.58% | 2.50% | 3.60% | 12.70% | 12.50% | p=0.003 | 22.09% | 20.00% | p=0.002 | |
| IV | N | 14 | 14 | 2 | 2 | NA | NA | 16 | 16 | 0.791 | 16 | 16 | 0.865 |
| Median | 36.93% | 54.60% | 31.55% | 41.86% | NA | NA | 13.05% | 10.00% | p=0.00 | 24.29% | 20.00% | p=0.000 | |
| Total | N | 50 | 50 | 50 | 50 | ||||||||
| Median | 13.05% | 10.00% | 24.29% | 20.00% | |||||||||