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. 2016 Nov 3;49(6):2275–2284. doi: 10.3892/ijo.2016.3757

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Copyright: © Oi et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Immunohistochemical analysis of cucurbitacin I-treated tumors. Representative 143B tumors of athymic nude mice were immunohistochemically analyzed on day 14 after administration of control (A, C, E, G, I, K, M, O and Q) or 1.0 mg/kg cucurbitacin I (B, D, F, H, J, L, N, P and R). (A and B) Hematoxylin-eosin (H&E) staining. (C and D) Immunohistochemical staining of phospho-STAT3. (E and F) p-cyclin D1. (G and H) c-Myc. (I and J) Cleaved PARP. (K and L) Suvivin. (M and N) Cleaved caspase-3. (O and P) TUNEL assay. (Q and R) Negative control.