Table 3. Basal concentration of peptide orexins and mouse β-endorphin (n=6).
Basal dialysate concentration was calculated by external calibration and corrected for dilution of dialysate by adding acetonitrile and FA. Basal extracellular concentration was estimated according to average in vitro recovery.
peptide | Basal ± SEM concentration in dialysate (pM) | Basal extracellular ± SEM concentration (pM) |
---|---|---|
Orexin A | 3.0±0.7 | 1500±350 |
Orexin B | 2.3±0.6 | 23±6 |
β-endorphin, mouse | 2.5±0.3 | 23±3 |