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. Author manuscript; available in PMC: 2017 Dec 1.
Published in final edited form as: Cell Microbiol. 2016 Jul 22;18(12):1857–1870. doi: 10.1111/cmi.12629

Figure 1. Cytotoxicity occurs in a bile concentration dependent manner.

Figure 1

AM-19226 T3SS WT was grown overnight in LB and used to infect Caco2-BBE cells at an MOI of ~10 in the presence of varying bile concentrations as indicated. After 3h or 5h of co-culture, lactate dehydrogenase (LDH) was measured in the co-culture supernatant as an indicator of membrane permeability. Percent cytotoxicity was calculated relative to a total lysis control sample. Data represent the mean ± standard deviation from one experiment using three individual bacterial colonies. The experiment was repeated twice with similar results.