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. Author manuscript; available in PMC: 2017 Dec 1.
Published in final edited form as: Cell Microbiol. 2016 Jul 22;18(12):1857–1870. doi: 10.1111/cmi.12629

Figure 2. Caco2-BBE PARP is not cleaved during AM-19226 co-culture.

Figure 2

AM-19226 T3SS WT and ΔvcsN2 strains were grown overnight in LB medium and used to infect Caco2-BBE cells at an MOI of ~10 in medium containing 0.2% bile. After 1h, 1.5h, or 2h of co-culture, Caco2-BBE protein lysates were harvested and immunoblotted with anti-poly(ADP-ribose) polymerase (PARP) and anti-GAPDH antibodies. Protein lysates were also harvested from mammalian cells treated with 2µM staurosporine (STS) for 14 hours to induce apoptosis. Experiments were repeated twice and yielded similar results.