. Author manuscript; available in PMC: 2017 Nov 1.

Published in final edited form as: Ann N Y Acad Sci. 2016 Aug 15;1383(1):80–87. doi: 10.1111/nyas.13206

Table 1.

Runx2-related miRNAs in skeleton

miRNA	Target gene	Study model	Function/activity
miR-23a~27a~24-2²⁰	SATB2, Runx2	Primary rat osteoblasts, mouse pre-osteoblast cell line MC3T3-E1	Suppress osteogenic differentiation
A panel of 11 miRNAs (miR-23a, -30c, -34c, -133a, -135a, -137, -204, -205, -217, -218, -338)²¹	Runx2	MC3T3-E1, mouse chondroprogenitor cell line ATDC5	Suppress osteogenic differentiation; promote early chondrogenic differentiation and suppress chondrocyte maturation
miR-204/211^{22, 37}	Runx2	Multiple mesenchymal cell lines, human MSCs, primary mouse bone marrow stromal cells; primary mouse vascular smooth muscle cells (VSMCs), aortic calcification mouse model	Suppress osteogenic differentiation, promote adipogenic differentiation; downregulated in calcified VSMCs, suppress VSMCs/artery calcification
miR-320²³	Runx2	Human MSCs	Suppress osteogenic differentiation, promote adipogenic differentiation
miR-2861~3960²⁴	HDAC5, Hoxa2	Primary mouse osteoblasts, mouse mesenchymal cell line ST2	Promote osteogenic differentiation
miR-22²⁵	HDAC6	Human adipose tissue–derived MSCs	Promote osteogenic differentiation, suppress adipogenic differentiation
miR-449a²⁶	HDAC1	Human-induced pluripotent stem cells (iPSCs)	Promote osteogenic differentiation
miR-455-3p²⁷	Runx2	ATDC5	Promote early chondrogenic differentiation and suppress chondrocyte maturation
miR-1²⁸	HDAC4	Primary chicken chondrocytes	Promote chondrocyte maturation
miR-338-3p²⁹	Runx2, Fgfr2	Ovariectomy-induced osteoporosis mice	Upregulated in osteoporosis mice
miR-29a³⁰	HDAC4	Transgenic mice overexpressing miR-29a	Mitigate glucocorticoid-induced osteoporosis
miR-34c³¹	Runx2	Human osteosarcoma cell line SAOS-2 and U2OS, human osteosarcoma biopsies	Downregulated in osteosarcoma
miR-23a³²	Runx2, CXCL12	Multiple osteosarcoma cell lines, osteosarcoma nude mice model	Downregulated in osteosarcoma cell lines, suppress osteosarcoma tumor growth in nude mice
miR-30a³³	Runx2	Multiple osteosarcoma cell lines	Downregulated in osteosarcoma cell lines
miR-203^{35, 36}	Runx2	Multiple prostate/breast cancer cell lines, bone metastasis mice model	Downregulated in prostate/breast cancer cell lines, suppress prostate/breast cancer metastasis in vivo
miR-135³⁶	Runx2	Multiple breast cancer cell lines, bone metastasis mice model	Downregulated in breast cancer cell lines, suppress breast cancer metastasis in vivo
miR-30b/c³⁸	Runx2	Human coronary artery smooth muscle cells (CASMCs)	Downregulated in calcified CASMCs and human coronary artery atherosclerosis
miR-133a³⁹	Runx2	Primary mouse VSMCs	Downregulated in calcified VSMCs
miR-205⁴⁰	Runx2	Human aortic smooth muscle cells (HASMCs)	Downregulated in calcified HASMCs
miR-105⁵³	Runx2	Primary human osteoarthritic chondrocytes/biopsies	Downregulated in osteoarthritis
miR-145⁵⁴	Sox9	Primary human healthy chondrocytes	Overexpressing miR-145 promotes Runx2 expression
miR-365^{55, 56}	HDAC4	Primary human osteoarthritic chondrocytes/biopsies	Upregulated in osteoarthritis