Table 3.
Folding free energy change upon mutations (kcal/mol). Positive value indicates that the mutation decreases domain stability. The most contradictory predictions are underlined and not used. The averaged prediction is shown in the last column.
| mCSM | SDM | DUET | SAAFEC | Average | |
|---|---|---|---|---|---|
| A77T | 0.88 | 2.48 | 0.83 | 1.44 | 1.41 |
| D87G | 0.84 | 1.9 | 0.86 | 5.01 | 2.16 |
| A388P | 0.44 | 2.12 | 0.57 | −0.13 | 0.75 |
| D402Y | 1.03 | −0.8 | 1.04 | −7.93 | 0.42 |
| S451R | 0.52 | 0.25 | 0.43 | −3.74 | 0.40 |
| V504M | 0.16 | 0.88 | 0.16 | −0.14 | 0.27 |
| F642L | 1.43 | 1.05 | 1.56 | 1.71 | 1.44 |
| E698K | 0.22 | 3.27 | 0.29 | 4.16 | 1.99 |
| L731F | 1.5 | 0.31 | 1.69 | 0.01 | 0.88 |
| R750W | −0.56 | −1.74 | 0.76 | 1.01 | 0.40 |
| Y751C | 1.66 | −1.76 | 1.57 | 1.44 | 1.56 |