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. Author manuscript; available in PMC: 2017 Nov 1.
Published in final edited form as: FEBS J. 2016 Oct 1;283(21):3898–3918. doi: 10.1111/febs.13895

Figure 1.

Figure 1

Efficacy of Gal/VNPT55 on PC-3 xenografts. (a) PC-3 cells were inoculated into the flanks of male SCID mice and treated with either 0.15 mmol/kg gal (12 mice) or vehicle (24 mice) b.i.d. Mice were evaluated daily for the formation of palpable tumor. (b) Male SCID mice were inoculated with PC-3 cells and treated with either vehicle (0.3% hydroxypropyl cellulose, HPC) or 0.15 mmol/kg/b.i.d. d gal. Tumors were measured with calipers as described in materials and methods. (c) Excised PC-3 tumors were weighed following two weeks of treatment. (d) 1 × 106 LNCaP and CWR22Rv1 cells were seeded in 10 cm plates in 5% charcoal dextran supplemented RPMI and subsequently treated with gal (1–5 μM) for duration of 72 h. Immunoblot analysis was utilized to evaluate the expression of ERSR markers. (e) Tumors samples from 4 mice in each treatment group of LAPC4 xenografts were excised and analyzed by western blot for relative expression of ERSR markers, average expression were determined by densitometry (*p<0.05). (f) Cell viability assays were performed in DU145, PC-3 and CWR22Rv1 cells comparing efficacies of gal, VNPT55 and CGP-57380.